p c jun ser243 antibody (Cell Signaling Technology Inc)
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p c jun ser243 antibody
P C Jun Ser243 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p+c+jun+ser243+antibody/Phospho-c-Jun+(Ser243)+Antibody/pmc12924953-50-0-17
Average 94 stars, based on 30 article reviews
P C Jun Ser243 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p+c+jun+ser243+antibody/Phospho-c-Jun+(Ser243)+Antibody/pmc12924953-50-0-17
Average 94 stars, based on 30 article reviews
p c jun ser243 antibody - by Bioz Stars,
2026-09
94/100 stars
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1) Product Images from "ULK2 suppresses glycolysis to attenuate cisplatin resistance in ovarian cancer organoid via c-Jun phosphorylation"
Article Title: ULK2 suppresses glycolysis to attenuate cisplatin resistance in ovarian cancer organoid via c-Jun phosphorylation
Journal: Science Progress
doi: 10.1177/00368504261421695
Figure Legend Snippet: ULK2 modulated c-Jun phosphorylation in ovarian cancer organoids. (A) Immunoblot analysis of phospho-c-Jun (Ser243) and c-Jun expression, with β-actin as loading control. (B) In vitro kinase assay to test the phosphorylation of c-Jun protein by ULK2 protein. (C-D) Quantification of ULK2 and c-Jun immunofluorescence signals was performed using IPP 6.0 software. E. ULK2 and c-Jun were examined by immunofluorescence. (F) Co-IP analysis indicated an interaction between ULK2 and c-Jun in chemoresistant ovarian cancer organoid. (G) Analysis of protein stability by cycloheximide (CHX) chase assay. Western blot of organoid lysates following treatment with CHX (50 μg/mL) for the indicated durations. (H) c-Jun was immunoprecipitated from chemoresistant ovarian cancer organoids and subsequently probed with an antiubiquitin antibody by Western blot. (I) Analysis of c-Jun mRNA expression by qRT-PCR. (J) c-Jun silencing reduced the cell viability of chemo-resistant ovarian cancer organoid. Organoids were treated with 8 μg/L cisplatin in different time intervals. (K) Assessment of glucose uptake following the knockdown of c-Jun. (L) Analysis of lactate production subsequent to c-Jun silencing. (M) Effect of c-Jun overexpression was verified by qRT-PCR. (N) The overexpression of c-Jun counteracted the decrease in cell viability caused by the upregulation of ULK2 in chemo-resistant ovarian cancer organoid. (O) Glucose uptake assay. (P) Effects of ULK2 overexpression and c-Jun S243A mutation on cell viability in chemoresistant ovarian cancer organoids. Organoids were treated with 8 μg/L cisplatin in different time intervals. Q. ULK2 overexpression suppresses glucose uptake, which is rescued by the c-Jun S243A mutation in chemoresistant ovarian cancer organoids. * p < .05; ** p < .01.
Techniques Used: Phospho-proteomics, Western Blot, Expressing, Control, In Vitro, Kinase Assay, Immunofluorescence, Software, Co-Immunoprecipitation Assay, Immunoprecipitation, Quantitative RT-PCR, Knockdown, Over Expression, Mutagenesis
Figure Legend Snippet: ULK2 overexpression potentiated cisplatin sensitivity in ovarian cancer in xenograft models. (A) Images of subcutaneous xenograft tumor models established in nude mice. (B) The graph displayed the tumor growth progression observed in the nude mouse subjects. (C) Evaluation of tumor weights was conducted. ** p < .01. (D) Immunohistochemistry was performed to assess the association of ULK2, c-Jun and its phosphorylation at Ser243 site. (E) Immunofluorescence analysis was executed to confirm the interaction between ULK2 and p-c-Jun (Ser243) within the subcutaneous xenograft tumors. (F) Quantification of p-c-Jun (Ser243) immunofluorescence signal was performed using IPP 6.0 software. (G) Association between ULK2 and c-Jun was tested by immunofluorescence in the mice models. (H) The immunofluorescence intensity of c-Jun was quantified in ovarian cancer organoids using IPP 6.0 software.
Techniques Used: Over Expression, Immunohistochemistry, Phospho-proteomics, Immunofluorescence, Software
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Phospho-proteomics:Article Title: ULK2 suppresses glycolysis to attenuate cisplatin resistance in ovarian cancer organoid via c-Jun phosphorylation Article Snippet: ULK2 antibody (PA5-22173) was procured from Thermo Fisher Scientific (Waltham, MA, USA).ULK2 antibody (PA5-22173) was procured from Thermo Fisher Scientific (Waltham, MA, USA).. Article Title: ULK2 suppresses glycolysis to attenuate cisplatin resistance in ovarian cancer organoid via c-Jun phosphorylation. Article Snippet: ULK2 antibody (PA5-22173) was procured from Thermo Fisher Scientific (Waltham, MA, USA).ULK2 antibody (PA5-22173) was procured from Thermo Fisher Scientific (Waltham, MA, USA).. Article Title: ULK2 suppresses glycolysis to attenuate cisplatin resistance in ovarian cancer organoid via c-Jun phosphorylation Article Snippet: Images were acquired using a Nikon fluorescence microscope.Images were acquired using a Nikon fluorescence microscope.. Article Title: ULK2 suppresses glycolysis to attenuate cisplatin resistance in ovarian cancer organoid via c-Jun phosphorylation. Article Snippet: Images were acquired using a Nikon fluorescencemicroscope.Images were acquired using a Nikon fluorescencemicroscope.. Western Blot:Article Title: ULK2 suppresses glycolysis to attenuate cisplatin resistance in ovarian cancer organoid via c-Jun phosphorylation Article Snippet: ULK2 antibody (PA5-22173) was procured from Thermo Fisher Scientific (Waltham, MA, USA).ULK2 antibody (PA5-22173) was procured from Thermo Fisher Scientific (Waltham, MA, USA).. Article Title: ULK2 suppresses glycolysis to attenuate cisplatin resistance in ovarian cancer organoid via c-Jun phosphorylation. Article Snippet: ULK2 antibody (PA5-22173) was procured from Thermo Fisher Scientific (Waltham, MA, USA).ULK2 antibody (PA5-22173) was procured from Thermo Fisher Scientific (Waltham, MA, USA).. Article Title: ULK2 suppresses glycolysis to attenuate cisplatin resistance in ovarian cancer organoid via c-Jun phosphorylation Article Snippet: Images were acquired using a Nikon fluorescence microscope.Images were acquired using a Nikon fluorescence microscope.. Article Title: ULK2 suppresses glycolysis to attenuate cisplatin resistance in ovarian cancer organoid via c-Jun phosphorylation. Article Snippet: Images were acquired using a Nikon fluorescencemicroscope.Images were acquired using a Nikon fluorescencemicroscope.. Expressing:Article Title: ULK2 suppresses glycolysis to attenuate cisplatin resistance in ovarian cancer organoid via c-Jun phosphorylation Article Snippet: ULK2 antibody (PA5-22173) was procured from Thermo Fisher Scientific (Waltham, MA, USA).ULK2 antibody (PA5-22173) was procured from Thermo Fisher Scientific (Waltham, MA, USA).. Article Title: ULK2 suppresses glycolysis to attenuate cisplatin resistance in ovarian cancer organoid via c-Jun phosphorylation. Article Snippet: ULK2 antibody (PA5-22173) was procured from Thermo Fisher Scientific (Waltham, MA, USA).ULK2 antibody (PA5-22173) was procured from Thermo Fisher Scientific (Waltham, MA, USA).. Article Title: ULK2 suppresses glycolysis to attenuate cisplatin resistance in ovarian cancer organoid via c-Jun phosphorylation Article Snippet: Images were acquired using a Nikon fluorescence microscope.Images were acquired using a Nikon fluorescence microscope.. Article Title: ULK2 suppresses glycolysis to attenuate cisplatin resistance in ovarian cancer organoid via c-Jun phosphorylation. Article Snippet: Images were acquired using a Nikon fluorescencemicroscope.Images were acquired using a Nikon fluorescencemicroscope.. Control:Article Title: ULK2 suppresses glycolysis to attenuate cisplatin resistance in ovarian cancer organoid via c-Jun phosphorylation Article Snippet: ULK2 antibody (PA5-22173) was procured from Thermo Fisher Scientific (Waltham, MA, USA).ULK2 antibody (PA5-22173) was procured from Thermo Fisher Scientific (Waltham, MA, USA).. Article Title: ULK2 suppresses glycolysis to attenuate cisplatin resistance in ovarian cancer organoid via c-Jun phosphorylation. Article Snippet: ULK2 antibody (PA5-22173) was procured from Thermo Fisher Scientific (Waltham, MA, USA).ULK2 antibody (PA5-22173) was procured from Thermo Fisher Scientific (Waltham, MA, USA).. Article Title: ULK2 suppresses glycolysis to attenuate cisplatin resistance in ovarian cancer organoid via c-Jun phosphorylation Article Snippet: Images were acquired using a Nikon fluorescence microscope.Images were acquired using a Nikon fluorescence microscope.. Article Title: ULK2 suppresses glycolysis to attenuate cisplatin resistance in ovarian cancer organoid via c-Jun phosphorylation. Article Snippet: Images were acquired using a Nikon fluorescencemicroscope.Images were acquired using a Nikon fluorescencemicroscope.. In Vitro:Article Title: ULK2 suppresses glycolysis to attenuate cisplatin resistance in ovarian cancer organoid via c-Jun phosphorylation Article Snippet: ULK2 antibody (PA5-22173) was procured from Thermo Fisher Scientific (Waltham, MA, USA).ULK2 antibody (PA5-22173) was procured from Thermo Fisher Scientific (Waltham, MA, USA).. Article Title: ULK2 suppresses glycolysis to attenuate cisplatin resistance in ovarian cancer organoid via c-Jun phosphorylation. Article Snippet: ULK2 antibody (PA5-22173) was procured from Thermo Fisher Scientific (Waltham, MA, USA).ULK2 antibody (PA5-22173) was procured from Thermo Fisher Scientific (Waltham, MA, USA).. Article Title: ULK2 suppresses glycolysis to attenuate cisplatin resistance in ovarian cancer organoid via c-Jun phosphorylation Article Snippet: Images were acquired using a Nikon fluorescence microscope.Images were acquired using a Nikon fluorescence microscope.. Article Title: ULK2 suppresses glycolysis to attenuate cisplatin resistance in ovarian cancer organoid via c-Jun phosphorylation. Article Snippet: Images were acquired using a Nikon fluorescencemicroscope.Images were acquired using a Nikon fluorescencemicroscope.. Kinase Assay:Article Title: ULK2 suppresses glycolysis to attenuate cisplatin resistance in ovarian cancer organoid via c-Jun phosphorylation Article Snippet: ULK2 antibody (PA5-22173) was procured from Thermo Fisher Scientific (Waltham, MA, USA).ULK2 antibody (PA5-22173) was procured from Thermo Fisher Scientific (Waltham, MA, USA).. Article Title: ULK2 suppresses glycolysis to attenuate cisplatin resistance in ovarian cancer organoid via c-Jun phosphorylation. Article Snippet: ULK2 antibody (PA5-22173) was procured from Thermo Fisher Scientific (Waltham, MA, USA).ULK2 antibody (PA5-22173) was procured from Thermo Fisher Scientific (Waltham, MA, USA).. Article Title: ULK2 suppresses glycolysis to attenuate cisplatin resistance in ovarian cancer organoid via c-Jun phosphorylation Article Snippet: Images were acquired using a Nikon fluorescence microscope.Images were acquired using a Nikon fluorescence microscope.. Article Title: ULK2 suppresses glycolysis to attenuate cisplatin resistance in ovarian cancer organoid via c-Jun phosphorylation. Article Snippet: Images were acquired using a Nikon fluorescencemicroscope.Images were acquired using a Nikon fluorescencemicroscope.. Immunofluorescence:Article Title: ULK2 suppresses glycolysis to attenuate cisplatin resistance in ovarian cancer organoid via c-Jun phosphorylation Article Snippet: ULK2 antibody (PA5-22173) was procured from Thermo Fisher Scientific (Waltham, MA, USA).ULK2 antibody (PA5-22173) was procured from Thermo Fisher Scientific (Waltham, MA, USA).. Article Title: ULK2 suppresses glycolysis to attenuate cisplatin resistance in ovarian cancer organoid via c-Jun phosphorylation. Article Snippet: ULK2 antibody (PA5-22173) was procured from Thermo Fisher Scientific (Waltham, MA, USA).ULK2 antibody (PA5-22173) was procured from Thermo Fisher Scientific (Waltham, MA, USA).. Article Title: ULK2 suppresses glycolysis to attenuate cisplatin resistance in ovarian cancer organoid via c-Jun phosphorylation Article Snippet: Images were acquired using a Nikon fluorescence microscope.Images were acquired using a Nikon fluorescence microscope.. Article Title: ULK2 suppresses glycolysis to attenuate cisplatin resistance in ovarian cancer organoid via c-Jun phosphorylation. Article Snippet: Images were acquired using a Nikon fluorescencemicroscope.Images were acquired using a Nikon fluorescencemicroscope.. Software:Article Title: ULK2 suppresses glycolysis to attenuate cisplatin resistance in ovarian cancer organoid via c-Jun phosphorylation Article Snippet: ULK2 antibody (PA5-22173) was procured from Thermo Fisher Scientific (Waltham, MA, USA).ULK2 antibody (PA5-22173) was procured from Thermo Fisher Scientific (Waltham, MA, USA).. Article Title: ULK2 suppresses glycolysis to attenuate cisplatin resistance in ovarian cancer organoid via c-Jun phosphorylation. Article Snippet: ULK2 antibody (PA5-22173) was procured from Thermo Fisher Scientific (Waltham, MA, USA).ULK2 antibody (PA5-22173) was procured from Thermo Fisher Scientific (Waltham, MA, USA).. Article Title: ULK2 suppresses glycolysis to attenuate cisplatin resistance in ovarian cancer organoid via c-Jun phosphorylation Article Snippet: Images were acquired using a Nikon fluorescence microscope.Images were acquired using a Nikon fluorescence microscope.. Article Title: ULK2 suppresses glycolysis to attenuate cisplatin resistance in ovarian cancer organoid via c-Jun phosphorylation. Article Snippet: Images were acquired using a Nikon fluorescencemicroscope.Images were acquired using a Nikon fluorescencemicroscope.. Co-Immunoprecipitation Assay:Article Title: ULK2 suppresses glycolysis to attenuate cisplatin resistance in ovarian cancer organoid via c-Jun phosphorylation Article Snippet: ULK2 antibody (PA5-22173) was procured from Thermo Fisher Scientific (Waltham, MA, USA).ULK2 antibody (PA5-22173) was procured from Thermo Fisher Scientific (Waltham, MA, USA).. Article Title: ULK2 suppresses glycolysis to attenuate cisplatin resistance in ovarian cancer organoid via c-Jun phosphorylation. Article Snippet: ULK2 antibody (PA5-22173) was procured from Thermo Fisher Scientific (Waltham, MA, USA).ULK2 antibody (PA5-22173) was procured from Thermo Fisher Scientific (Waltham, MA, USA).. Article Title: ULK2 suppresses glycolysis to attenuate cisplatin resistance in ovarian cancer organoid via c-Jun phosphorylation Article Snippet: Images were acquired using a Nikon fluorescence microscope.Images were acquired using a Nikon fluorescence microscope.. Article Title: ULK2 suppresses glycolysis to attenuate cisplatin resistance in ovarian cancer organoid via c-Jun phosphorylation. Article Snippet: Images were acquired using a Nikon fluorescencemicroscope.Images were acquired using a Nikon fluorescencemicroscope.. 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Article Title: ULK2 suppresses glycolysis to attenuate cisplatin resistance in ovarian cancer organoid via c-Jun phosphorylation. Article Snippet: Images were acquired using a Nikon fluorescencemicroscope.Images were acquired using a Nikon fluorescencemicroscope.. Quantitative RT-PCR:Article Title: ULK2 suppresses glycolysis to attenuate cisplatin resistance in ovarian cancer organoid via c-Jun phosphorylation Article Snippet: ULK2 antibody (PA5-22173) was procured from Thermo Fisher Scientific (Waltham, MA, USA).ULK2 antibody (PA5-22173) was procured from Thermo Fisher Scientific (Waltham, MA, USA).. Article Title: ULK2 suppresses glycolysis to attenuate cisplatin resistance in ovarian cancer organoid via c-Jun phosphorylation. Article Snippet: ULK2 antibody (PA5-22173) was procured from Thermo Fisher Scientific (Waltham, MA, USA).ULK2 antibody (PA5-22173) was procured from Thermo Fisher Scientific (Waltham, MA, USA).. Article Title: ULK2 suppresses glycolysis to attenuate cisplatin resistance in ovarian cancer organoid via c-Jun phosphorylation Article Snippet: Images were acquired using a Nikon fluorescence microscope.Images were acquired using a Nikon fluorescence microscope.. Article Title: ULK2 suppresses glycolysis to attenuate cisplatin resistance in ovarian cancer organoid via c-Jun phosphorylation. Article Snippet: Images were acquired using a Nikon fluorescencemicroscope.Images were acquired using a Nikon fluorescencemicroscope.. Knockdown:Article Title: ULK2 suppresses glycolysis to attenuate cisplatin resistance in ovarian cancer organoid via c-Jun phosphorylation Article Snippet: ULK2 antibody (PA5-22173) was procured from Thermo Fisher Scientific (Waltham, MA, USA).ULK2 antibody (PA5-22173) was procured from Thermo Fisher Scientific (Waltham, MA, USA).. Article Title: ULK2 suppresses glycolysis to attenuate cisplatin resistance in ovarian cancer organoid via c-Jun phosphorylation. Article Snippet: ULK2 antibody (PA5-22173) was procured from Thermo Fisher Scientific (Waltham, MA, USA).ULK2 antibody (PA5-22173) was procured from Thermo Fisher Scientific (Waltham, MA, USA).. Article Title: ULK2 suppresses glycolysis to attenuate cisplatin resistance in ovarian cancer organoid via c-Jun phosphorylation Article Snippet: Images were acquired using a Nikon fluorescence microscope.Images were acquired using a Nikon fluorescence microscope.. Article Title: ULK2 suppresses glycolysis to attenuate cisplatin resistance in ovarian cancer organoid via c-Jun phosphorylation. Article Snippet: Images were acquired using a Nikon fluorescencemicroscope.Images were acquired using a Nikon fluorescencemicroscope.. Over Expression:Article Title: ULK2 suppresses glycolysis to attenuate cisplatin resistance in ovarian cancer organoid via c-Jun phosphorylation Article Snippet: ULK2 antibody (PA5-22173) was procured from Thermo Fisher Scientific (Waltham, MA, USA).ULK2 antibody (PA5-22173) was procured from Thermo Fisher Scientific (Waltham, MA, USA).. Article Title: ULK2 suppresses glycolysis to attenuate cisplatin resistance in ovarian cancer organoid via c-Jun phosphorylation. Article Snippet: ULK2 antibody (PA5-22173) was procured from Thermo Fisher Scientific (Waltham, MA, USA).ULK2 antibody (PA5-22173) was procured from Thermo Fisher Scientific (Waltham, MA, USA).. Article Title: ULK2 suppresses glycolysis to attenuate cisplatin resistance in ovarian cancer organoid via c-Jun phosphorylation Article Snippet: Images were acquired using a Nikon fluorescence microscope.Images were acquired using a Nikon fluorescence microscope.. Article Title: ULK2 suppresses glycolysis to attenuate cisplatin resistance in ovarian cancer organoid via c-Jun phosphorylation. Article Snippet: Images were acquired using a Nikon fluorescencemicroscope.Images were acquired using a Nikon fluorescencemicroscope.. Mutagenesis:Article Title: ULK2 suppresses glycolysis to attenuate cisplatin resistance in ovarian cancer organoid via c-Jun phosphorylation Article Snippet: ULK2 antibody (PA5-22173) was procured from Thermo Fisher Scientific (Waltham, MA, USA).ULK2 antibody (PA5-22173) was procured from Thermo Fisher Scientific (Waltham, MA, USA).. Article Title: ULK2 suppresses glycolysis to attenuate cisplatin resistance in ovarian cancer organoid via c-Jun phosphorylation. Article Snippet: ULK2 antibody (PA5-22173) was procured from Thermo Fisher Scientific (Waltham, MA, USA).ULK2 antibody (PA5-22173) was procured from Thermo Fisher Scientific (Waltham, MA, USA).. Article Title: ULK2 suppresses glycolysis to attenuate cisplatin resistance in ovarian cancer organoid via c-Jun phosphorylation Article Snippet: Images were acquired using a Nikon fluorescence microscope.Images were acquired using a Nikon fluorescence microscope.. Article Title: ULK2 suppresses glycolysis to attenuate cisplatin resistance in ovarian cancer organoid via c-Jun phosphorylation. Article Snippet: Images were acquired using a Nikon fluorescencemicroscope.Images were acquired using a Nikon fluorescencemicroscope.. Immunohistochemistry:Article Title: ULK2 suppresses glycolysis to attenuate cisplatin resistance in ovarian cancer organoid via c-Jun phosphorylation Article Snippet: ULK2 antibody (PA5-22173) was procured from Thermo Fisher Scientific (Waltham, MA, USA).ULK2 antibody (PA5-22173) was procured from Thermo Fisher Scientific (Waltham, MA, USA).. Article Title: ULK2 suppresses glycolysis to attenuate cisplatin resistance in ovarian cancer organoid via c-Jun phosphorylation. Article Snippet: ULK2 antibody (PA5-22173) was procured from Thermo Fisher Scientific (Waltham, MA, USA).ULK2 antibody (PA5-22173) was procured from Thermo Fisher Scientific (Waltham, MA, USA).. Article Title: ULK2 suppresses glycolysis to attenuate cisplatin resistance in ovarian cancer organoid via c-Jun phosphorylation Article Snippet: Images were acquired using a Nikon fluorescence microscope.Images were acquired using a Nikon fluorescence microscope.. Article Title: ULK2 suppresses glycolysis to attenuate cisplatin resistance in ovarian cancer organoid via c-Jun phosphorylation. Article Snippet: Images were acquired using a Nikon fluorescencemicroscope.Images were acquired using a Nikon fluorescencemicroscope.. |